Application

Anti-Omentin Antibody detects level of Omentin & has been published & validated for use in ELISA & WB.

Western blot: 1:2,000-1:5,000 using ECL. Reacted with the ~40kDa Omentin His-tagged protein. Suggested blocking and dilution buffer is PBST with 0.05% Tween-20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature.

ELISA, direct and indirect: 1:2,000-1:5,000.

General description

A calcium-dependent lectin with affinity for furanosides, such as galactofuranosyl in bacterial arabinogalactans. 30kD of apparent molecular weight of Omentin migrate at 40-kD under reducing conditions due to glycosylation. Omentin, a novel visceral fat depot-specific secretory protein, enhance insulin-stimulated glucose transport in subcutaneous as well as omental adipocytes and may therefore be implicated in the pathogenesis of obesity.

Immunogen

Epitope: Amino acids 95-115 of Human Omentin.

Synthetic peptide: TVGDRWSSQQGSKADYPEGDG, corresponding to amino acids 95-115 of Human Omentin.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Liquid in PBS, pH7.4. Contains no preservatives.

Specificity

Recognizes Omentin in western blotting.

Target description

30-40 kDa